SC5b-9 Level - Terminal Complement Complex (Urine)
Test Code
SC59U
Description
Complement mediated renal diseases such as C3 glomerulopathy (C3G), atypical hemolytic uremic syndrome (aHUS), lupus nephritis (LN) and immunoglobulin A nephropathy (IgAN) are characterized by dysregulated complement activation and increased complement activation products in urine, including soluble C5b-9 (sC5b-9), a marker of terminal complement activation.
The sC5b-9 protein levels measured in urine are reported as a ratio to the urine creatinine levels. While sC5b-9 levels are undetectable in urine from healthy individuals, it can be elevated in many inflammatory kidney conditions.
Collect
Urine collected via clean-catch midstream technique at first void in a sterile urine cup; place on ice ASAP following collection. Transfer urine to clean centrifuge tube on ice.
Patient Prep
Centrifuge tube at 600 (± 50) x g (rcf) at 4°C (±2) for 15 (±1) minutes. Transfer the urine to centrifuged urine to a clean cryogenic storage tube, and immediately store the cryogenic tube at -20°C, or at -60°C or below in an ultra-low freezer.
Preferred volume: 1.0 mL urine
Minimum volume: 0.5 mL urine
Pediatric Collection
Minimum volume: 0.5 mL urine
Unacceptable Conditions
Thawed samples
Storage Transport Temp
Send samples overnight in a well-insulated container on dry ice.
Stability
-20°C - up to 4 weeks
-60°C or below in an ultra-low freezer - up to 2 years
Notes
Additional testing may be needed for less than perfect samples on confirmatory testing/reflex testing.
To prevent unnecessary delays in testing, please send one aliquot per test request.
Performed
Monday - Friday
Methodology
ELISA (Urine sC5b9)
Modified Rate Jaffe (Urine Creatinine)
Turnaround Time
Up to 3 weeks
Department
Complement Lab
Synonyms
Terminal Complement Complex (TCC), ELISA (SC5b-9 Level – terminal complement complex), Complement (SC5b-9 Level – terminal complement complex), Complement Urine; Soluble C5b-9 Complex, Membrane Attack Complex (MAC)
Study Offerings
CAP/CLIA
Interpretation
- Result reported as the ratio of urine sC5b9 (ng) to urine creatinine (mg)
- Ratio in normal, healthy urine is non-detectable. A positive ratio indicates the presence of urine sC5b9.
CPT Code
86160
New York Approved
No: Run with Waiver
FDA
LDT